SMHS SciVisualization

Questions

• How and why should we “look” at data?

• What data characteristics are important for exploratory data analytics (EDAs)?

Scientific Data-driven or Simulation-driven visualization methods may be classified in many alternative ways. Visualization techniques can be classified according to many criteria:

• Data Type: structured/unstructured, small/large, complete/incomplete, time/space, ascii/binary, Euclidean/non-Euclidean, etc.

• Task type: Task type is one of the aspects considered in classification of visualization techniques, which provides means of interaction between the researcher, the data and the display software/platform

• Scalability: Visualization techniques are subject to some limitations, such as the amount of data that a particular technique can exhibit

• Dimensionality: Visualization techniques can also be classified according to the number of attributes

• Positioning and Attributes: the distribution of attributes on the chart may affect the interpretation of the display representation, e.g., correlation analysis, where the relative distance among the plotted attributes is relevant for observation

• Investigative Need: the specific scientific question or exploratory interest may also determine the type of visualization:

o Examining the composition of the data

o Exploring the distribution of the data

o Contrasting or comparing several data elements, relations, association

o Unsupervised exploratory data mining

http://www.socr.umich.edu/CSCD/html/Cores/Macore2/SciViz.html

SOCR Charts

• URL: http://socr.umich.edu/html/cha/ (Java applet)

• About/List: http://wiki.stat.ucla.edu/socr/index.php/About_pages_for_SOCR_Chart_List

• Activities: http://wiki.stat.ucla.edu/socr/index.php/SOCR_EduMaterials_ChartsActivities

• Data: http://wiki.socr.umich.edu/index.php/SOCR_Data

Excel Charts

MS Excel provides a large number of charts that can be used to quickly and effectively render complex multivariate data. For instance, the table below contains the principal component analysis (PCA) of 50 derived neuroimaging biomarkers (region of interest (ROI) by shape morphometry metric). The loadings of these 50 variables on the top 5 (most significant) principal component directions are shown in the table. Experiment with effective visualizations of these data.

Hemi	ROI	measure	ROI_Measure	Dim.1	Dim.2	Dim.3	Dim.4	Dim.5
L	insular	AvgMeanCurvature	L_insular_cortex_AvgMeanCurvature	0.72	0	0.06	0.06	0
L	insular	ComputeArea	L_insular_cortex_ComputeArea	0.77	0.06	0.04	0.01	0
L	insular	Volume	L_insular_cortex_Volume	0.72	0.09	0.04	0.03	0.01
L	insular	ShapeIndex	L_insular_cortex_ShapeIndex	0.46	0.06	0.01	0.02	0.01
L	insular	Curvedness	L_insular_cortex_Curvedness	0.78	0	0.05	0.03	0.01
R	insular	AvgMeanCurvature	R_insular_cortex_AvgMeanCurvature	0.79	0	0.03	0.08	0
R	insular	ComputeArea	R_insular_cortex_ComputeArea	0.79	0.09	0.03	0.01	0
R	insular	Volume	R_insular_cortex_Volume	0.73	0.11	0.03	0.03	0
R	insular	ShapeIndex	R_insular_cortex_ShapeIndex	0.27	0.17	0	0.07	0
R	insular	Curvedness	R_insular_cortex_Curvedness	0.84	0.02	0.03	0.01	0
L	cingulate	AvgMeanCurvature	L_cingulate_gyrus_AvgMeanCurvature	0.72	0	0.05	0.02	0.02
L	cingulate	ComputeArea	L_cingulate_gyrus_ComputeArea	0.74	0.05	0.06	0.04	0.01
L	cingulate	Volume	L_cingulate_gyrus_Volume	0.69	0.08	0.05	0.05	0.01
L	cingulate	ShapeIndex	L_cingulate_gyrus_ShapeIndex	0.53	0	0.05	0	0.03
L	cingulate	Curvedness	L_cingulate_gyrus_Curvedness	0.7	0.01	0.05	0.04	0.03
R	cingulate	AvgMeanCurvature	R_cingulate_gyrus_AvgMeanCurvature	0.6	0	0.02	0.03	0.01
R	cingulate	ComputeArea	R_cingulate_gyrus_ComputeArea	0.73	0.06	0.04	0.03	0.01
R	cingulate	Volume	R_cingulate_gyrus_Volume	0.68	0.09	0.04	0.04	0.01
R	cingulate	ShapeIndex	R_cingulate_gyrus_ShapeIndex	0.56	0.01	0.05	0	0.01
R	cingulate	Curvedness	R_cingulate_gyrus_Curvedness	0.25	0	0.01	0.04	0
L	caudate	AvgMeanCurvature	L_caudate_AvgMeanCurvature	0.52	0	0.05	0	0.01
L	caudate	ComputeArea	L_caudate_ComputeArea	0.51	0.09	0.03	0.04	0.02
L	caudate	Volume	L_caudate_Volume	0.44	0.09	0.03	0.06	0.03
L	caudate	ShapeIndex	L_caudate_ShapeIndex	0.2	0.03	0.04	0.04	0
L	caudate	Curvedness	L_caudate_Curvedness	0.51	0.12	0.02	0.01	0.01
R	caudate	AvgMeanCurvature	R_caudate_AvgMeanCurvature	0.68	0.04	0.04	0.02	0
R	caudate	ComputeArea	R_caudate_ComputeArea	0.67	0.17	0.03	0.02	0.01
R	caudate	Volume	R_caudate_Volume	0.61	0.16	0.02	0.03	0.01
R	caudate	ShapeIndex	R_caudate_ShapeIndex	0.18	0.02	0.03	0.11	0
R	caudate	Curvedness	R_caudate_Curvedness	0.65	0.19	0.01	0	0
L	putamen	AvgMeanCurvature	L_putamen_AvgMeanCurvature	0.62	0	0.04	0.03	0.02
L	putamen	ComputeArea	L_putamen_ComputeArea	0.56	0.05	0.04	0.03	0.05
L	putamen	Volume	L_putamen_Volume	0.52	0.07	0.04	0.05	0.05
L	putamen	ShapeIndex	L_putamen_ShapeIndex	0.06	0.13	0	0.15	0
L	putamen	Curvedness	L_putamen_Curvedness	0.64	0.11	0.03	0.01	0.03
R	putamen	AvgMeanCurvature	R_putamen_AvgMeanCurvature	0.62	0	0.07	0.04	0.01
R	putamen	ComputeArea	R_putamen_ComputeArea	0.66	0.08	0.03	0.01	0.03
R	putamen	Volume	R_putamen_Volume	0.64	0.12	0.03	0.02	0.03
R	putamen	ShapeIndex	R_putamen_ShapeIndex	0.15	0.24	0	0.08	0.03
R	putamen	Curvedness	R_putamen_Curvedness	0.65	0.05	0.05	0	0.02
L	hippocampus	AvgMeanCurvature	L_hippocampus_AvgMeanCurvature	0.78	0	0.01	0.04	0
L	hippocampus	ComputeArea	L_hippocampus_ComputeArea	0.75	0.07	0.01	0	0.02
L	hippocampus	Volume	L_hippocampus_Volume	0.72	0.09	0.01	0.01	0.01
L	hippocampus	ShapeIndex	L_hippocampus_ShapeIndex	0.45	0.17	0	0.04	0.02
L	hippocampus	Curvedness	L_hippocampus_Curvedness	0.79	0.03	0.01	0	0.02
R	hippocampus	AvgMeanCurvature	R_hippocampus_AvgMeanCurvature	0.72	0	0	0.1	0.01
R	hippocampus	ComputeArea	R_hippocampus_ComputeArea	0.71	0.09	0	0	0.05
R	hippocampus	Volume	R_hippocampus_Volume	0.68	0.1	0	0	0.04
R	hippocampus	ShapeIndex	R_hippocampus_ShapeIndex	0.37	0.18	0	0.02	0.03
R	hippocampus	Curvedness	R_hippocampus_Curvedness	0.77	0.03	0	0.02	0.04

R-Charts

There are 100’s of packages and 1,000 of different charts, plots and graphs that can be generated using R. Such interactive visualizations enable deeper exploration of data, models and results. JavaScript libraries, e.g., D3, provide advantages for data visualization as these involve HTML5 and are easily shareable online. The R community is developing R interfaces to some popular JavaScript libraries to allow users to create interactive visualizations without detailed knowledge of JavaScript.

Examples of powerful R interactive visualization packages

• ggplot2 – http://ggplot2.org

• ggvis – interactive plots extending the static ggplot2 charts, http://ggvis.rstudio.com

• rCharts – R interface to multiple JavaScript charting libraries, http://rcharts.io

• plotly – transforming ggplot2 charts into interactive plots, https://plot.ly/r

• googleVis – Google Charts using R, http://cran.r-project.org/web/packages/googleVis/vignettes/googleVis_examples.html

• HTMLWidgets

o leaflet – library for creating dynamic maps, supports panning and zooming, annotations, markers, polygons, etc. http://www.htmlwidgets.org/showcase_leaflet.html

o dygraphs – provides mechanism for charting time-series data, supports interactive navigation features including series/point highlighting, zooming, and panning, http://www.htmlwidgets.org/showcase_dygraphs.html

o networkD3 – library for creating D3 network graphs including force directed networks, Sankey diagrams, and Reingold-Tilford tree networks, http://www.htmlwidgets.org/showcase_networkD3.html

o DataTables – displays R matrices or data frames as interactive HTML tables that support filtering, pagination, and sorting, http://www.htmlwidgets.org/showcase_datatables.html

o Rthreejs – features 3D scatterplots and globes based on WebGL, http://www.htmlwidgets.org/showcase_threejs.html

• Other R graphic examples

o To write out plots out to file use:

# pdf() command all graphs are redirected to test.pdf.  Also works with other common formats:  jpeg, png, ps, tiff.
pdf("C:\\Users\\Dinov\\Desktop\\test.pdf"); plot(1:100, 1:100); dev.off()
# Generates Scalable Vector Graphics (SVG) that can be edited by vector graphics software
svg("test.svg"); plot(1:100, 1:100); dev.off()

Paired ScatterPlots

set.seed(100)
x <- matrix(runif(50), ncol=5, dimnames=list(letters[1:10], LETTERS[1:5]))
describe(x)    # library("Hmisc")
plot(x[,1], x[,2], pch=20, col="red", main="Symbols and Labels")
text(x[,1]+0.03, x[,2], rownames(x))

pairs(x)

Another way to generate scatterplots is by using ggplot:

# library(ggplot2)
x <- sample(1:20, 20); y <- sample(1:20, 20); cat <- rep(c("A", "B", "C", "D"), 5)  
#vs. cat <- rep(c("A", "B", "C", "D"), each=5)
plot.1 <- qplot(x, y, geom="point", size=5*x, color=cat, main="GGplot with Relative Dot Size and Color") + theme(legend.position = "topleft")
print(plot.1)

# Use Case-Studies: https://umich.instructure.com/courses/38100/files/folder/Case_Studies
#  Case_03_MentalHealthServicesSurvey
# data1 <- read.table('https://umich.instructure.com/files/399128/download?download_frd=1&verifier=AG2e9QUKUm1jvDBpkX7D9jbEjKNc4irA0ECk0f7p', header=T)	
head(data1)
attach(data1)
# library("Hmisc")
describe(data1)

plot(data1[,3], data1[,4], pch=20, col="red", main="Symbols and Labels")
# text(data1 [,3]+0.03, data1 [,4], rownames(data1))
plot.1 <- qplot(x, y, geom="point", size=5*x, color=cat, main="GGplot with Relative Dot Size and Color") + theme(legend.position = "topleft")
print(plot.1)

# redo plots using majorfundtype FacilityType Ownership Focus
# pairs(data1, na.action=na.omit)

# Scatterplot with regression line. Use the “diamonds” dataset, which is a data frame with
# 53,940 rows and 10 variables ()
# describe(diamonds)

# Use Case-Studies: https://umich.instructure.com/courses/38100/files/folder/Case_Studies
# CaseStudy01_Divorce_YoungAdults
# data1 <- read.csv('https://umich.instructure.com/files/399118/download?download_frd=1&verifier=ESACv31KcyiHbkPZPuT8Oo4V7XzPtgTTbs6PQLTv', header=T)	
attach(data1)
# plot variables: DIVYEAR momint dadint momclose depression livewithmom gethitched

set.seed(110)
# par(mfrow=c(1,2))
data.2 <- diamonds[sample(nrow(diamonds), 500), ]
plot.2 <- qplot(price, depth, data = data.2, geom = c("point", "smooth"), method = "lm")
plot.3 <- qplot(carat, price, data=data.2, geom=c("point", "smooth"), span=0.4)
print(plot.2); print(plot.3)

Barplots

x <- matrix(runif(50), ncol=5, dimnames=list(letters[1:10], LETTERS[1:5]))
barplot(x[1:4,], ylim=c(0, max(x[1:4,])+0.3), beside=TRUE, legend.text = letters[1:4],
       args.legend = list(x = "topleft"))
text(labels=round(as.vector(as.matrix(x[1:4,])),2), x=seq(1.5, 21, by=1) + sort(rep(c(0,1,2,3,4), 4)), y=as.vector(as.matrix(x[1:4,]))+0.1)

# to put error bars on barplot:

# 10 rows (a, b, c, …):
bar <- barplot(m <- rowMeans(x) * 10, ylim=c(0, 10))
stdev <- sd(t(x))
arrows(bar, m, bar, m + stdev, length=0.15, angle = 90)

# Case_04_ChildTrauma
# data1 <- read.table('https://umich.instructure.com/files/399129/download?download_frd=1&verifier=Hmv0YW2Kie5ZTV9CKBUNArSHR66f3GWSmVzZDBxc', header=T)	
attach(data1)
head(x)
head(data1)
# plot data
data2 <- data1[,-5]   # remove the 5th columns text
data1 <- data2[,-5]   # remove the 6th columns text
# or data1 <- data1[,c(-5,-6)]

data2 <- as.data.frame(data1)
Blacks <- data2[which(data2$\$$race=="black"),]
 Other <- data2[which(data2$\$$race=="other"),]
Hispanic <- data2[which(data2$\$$race=="hispanic"),]
 White <- data2[which(data2$\$$race=="white"),]

A <- c(mean(Blacks$\$$age), mean(Blacks$\$$service))
#colnames(A) <- c("age "," service ")  
B <- c(mean(Other$\$$age), mean(Other$\$$service))
C <- c(mean(Hispanic$\$$age), mean(Hispanic$\$$service))
D <- c(mean(White$\$$age), mean(White$\$$service))

x <- cbind(A, B, C, D)

bar <- barplot(x[1:2,], ylim=c(0, max(x[1:2,])+2.0), beside=TRUE, 
legend.text = c("age","service") ,  args.legend = list(x = "right"))
text(labels=round(as.vector(as.matrix(x[1:2,])),2), x=seq(1.4, 21, by=1.5), #y=as.vector(as.matrix(x[1:2,]))+0.3)

y=11.5)

m <- x; stdev <- sd(t(x))
arrows(bar, m, bar, m + stdev, length=0.15, angle = 90)

barplot(as.matrix(data1[1:4,]), ylim=c(0, max(data1[1:4,])+0.3), beside=TRUE, legend.text = data1[1:4,1], args.legend = list(x = "topleft"))
text(labels=round(as.vector(as.matrix(data1[1:4,])),2), x=seq(1.5, 21, by=1), y=as.vector(as.matrix(data1[1:4,]))+0.1)

# Columns (A, B, C, D, E):
bar <- barplot(m <- colMeans(x) * 5, ylim=c(0, 5))
stdev <- sd(t(x))
arrows(bar, m, bar, m + stdev, length=0.15, angle = 90)

Histograms and Density Plots

hist(x, freq=TRUE, breaks=10)

plot(density(x), lwd = 10, col="green")

Pie Chart

# first , “A”, and second, “B”, columns
par (mfrow=c(1,2))
pie(x[,1], col=rainbow(length(x[,1]), start=0.1, end=0.8), clockwise=TRUE)

pie(x[,1], col=rainbow(length(x[,1]), start=0.1, end=0.8), clockwise=TRUE)

pie(x[,2], col=rainbow(length(x[,2]), start=0.1, end=0.8), clockwise=TRUE)
legend("topleft", legend=row.names(x), cex=1.3, bty="n", pch=15, pt.cex=1.8, col=rainbow(length(x[,2]), start=0.1, end=0.8), ncol=1)

You can export the data: 
write.table(x, " ", "data.txt")
# copy-paste it in SOCR Pie chart to generate another Pie view of data

Line Plots Using ggplot

head(diamonds)

	Carat	Cut	Color	Clarity	Depth	Table	Price	X	Y	Z
1	0.23	Ideal	E	SI2	61.5	55	326	3.95	3.98	2.43
2	0.21	Premium	E	SI1	59.8	61	326	3.89	3.84	2.31
3	0.23	Good	E	VS1	56.9	65	237	4.05	4.07	2.31
4	0.29	Premium	I	VS2	62.4	58	334	4.2	4.23	2.63
5	0.31	Good	J	SI2	63.3	58	335	4.34	4.35	4.75
6	0.24	VeryGood	J	VVS2	62.8	57	336	3.94	3.96	2.48

plot.2 <- ggplot(diamonds, aes(carat, price, group=cut, color=cut)) + geom_line()
print(plot.2)

plot.2 <- ggplot(data1, aes(age, service, group=race, color=race)) + geom_line()
print(plot.2)

# Faceting plot (geometrically, faceting (or facetting) is the process of removing parts of a polygon, polyhedron or polytope, without creating any new vertices)
plot.3 <- ggplot(diamonds, aes(carat, price)) + geom_line(aes(color=cut), size=1) + 
facet_wrap(~cut, ncol=1)
print(plot.3)

Barplots with ggplot

plot.4 <- ggplot(diamonds, aes(cut, fill=cut)) + geom_bar() + facet_grid(. ~ clarity)
print(plot.4)

New_var <- service+rnorm(1000, 0,1)
data1$\$$New_var <- int(New_var)
plot.4 <- ggplot(data1, aes(race, fill= traumatype)) + geom_bar() + facet_grid(. ~ New_var)
print(plot.4)

plot.4a <- ggplot(diamonds, aes(color, price/carat, fill=color)) + geom_boxplot()
print(plot.4a)

Jitter plot

plot.5 <- ggplot(diamonds, aes(color, price/carat)) + geom_jitter(alpha = I(1 / 2), aes(color=color))
print(plot.5)

Density Plots

plot.6 <- ggplot(diamonds, aes(carat, size=2)) + geom_density(aes(color = cut))
print(plot.6)

plot.6 <- ggplot(data1, aes(age, size=2)) + geom_density(aes(color = traumatype))
print(plot.6)

plot.7 <- ggplot(diamonds, aes(carat, size=2)) + geom_density(aes(fill = color))
print(plot.7)

plot.8 <- ggplot(diamonds, aes(x=carat, size=1)) + geom_histogram(aes(y = price), binwidth=0.2) + geom_density()
print(plot.8)

plot.8a <- ggplot(diamonds, aes(x=carat, size=1)) + geom_histogram(aes(y = price), stat="identity") + geom_density()
print(plot.8a)

Heatmaps

# Generating Dendogram Association Heatmap Plot (Genotype vs. Imaging phenotype
# http://stat.ethz.ch/R-manual/R-patched/library/stats/html/heatmap.html
# http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4005931/ 

AD_Associations_Data <- read.table("https://umich.instructure.com/files/330387/download?download_frd=1&verifier=gLk2ADgrLhXGeknI6mqIeJugi2ODr8RARsQlBUMe", header=TRUE, row.names=1,  sep=",", dec=".")	   

MCI_Associations_Data <- read.table("https://umich.instructure.com/files/330390/download?download_frd=1&verifier=FczlJD6ISRPZhu69xvHuoZHx2c7gXX9YEvvPCTBG", header=TRUE, row.names=1,  sep=",", dec=".")	   	   

NC_Associations_Data <- read.table("https://umich.instructure.com/files/330391/download?download_frd=1&verifier=i2BEtSpmpbrzQUPoA2ST06IzzcaenyVEHRepHSF3", header=TRUE, row.names=1,  sep=",", dec=".")	   	   

require(graphics)
require(grDevices)
library(gplots)

AD_Data <- AD_Associations_Data 
MCI_Data <- MCI_Associations_Data 
NC_Data <- NC_Associations_Data 

AD_mat  <- as.matrix(AD_Data); class(AD_mat) <- "numeric"
MCI_mat  <- as.matrix(MCI_Data); class(MCI_mat) <- "numeric"
NC_mat  <- as.matrix(NC_Data); class(NC_mat) <- "numeric"

# set up the rol (rc) and column (cc) colors for each cohort
rcAD <- rainbow(nrow(AD_mat), start = 0, end = 1.0); ccAD<-rainbow(ncol(AD_mat), start = 0, end = 1.0)
rcMCI <- rainbow(nrow(MCI_mat), start = 0, end=1.0); ccMCI<-rainbow(ncol(MCI_mat),start=0,end=1.0)
rcNC <- rainbow(nrow(NC_mat), start = 0, end = 1.0); ccNC<-rainbow(ncol(NC_mat), start = 0, end = 1.0)

# set up 1x3 graph display - par (mfrow=c(1,3)) – does not work with ‘heatmap’
hvAD <- heatmap(AD_mat, col = cm.colors(256), scale = "column", RowSideColors = rcAD, ColSideColors = ccAD, margins = c(2,2), main="AD Cohort SNP-ROI_volume Association (p_values)")
hvMCI <- heatmap(MCI_mat, col = cm.colors(256), scale = "column", RowSideColors = rcMCI, ColSideColors = ccMCI, margins = c(2,2), main="MCI Cohort SNP-ROI_volume Association (p_values)")
hvNC <- heatmap(NC_mat, col = cm.colors(256), scale = "column", RowSideColors = rcNC, ColSideColors = ccNC, margins = c(2,2), main="NC Cohort SNP-ROI_volume Association (p_values)")

# Alternatively, we can use the R package gplots
install.packages("gplots")
library(gplots)
## col dendrogram plotted and col reordering done. 
# heatmap.2(AD_mat, keysize=2) 
## A more decorative heatmap, with z-score scaling along columns 
heatmap.2(AD_mat, col=cm.colors(255), scale="column", RowSideColors=rcAD, ColSideColors=ccAD, margin=c(8, 7), xlab="Imaging Biomarkers (ROI volume)", ylab= "Genetics Biomarkers (SNPs)", main="AD Associations Heatmap (SNP-Imaging)",     tracecol="green", density="density")

Correlation Plots

The corrplot package is a graphical display of a correlation matrix, and confidence intervals, with some tools for matrix reordering. There are seven visualization methods (parameter method) in corrplot package, named   "circle", "square", "ellipse", "number", "shade", "color", "pie".
# install.packages("corrplot")
library(corrplot)
NC_Associations_Data <- read.table("https://umich.instructure.com/files/330391/download?download_frd=1&verifier=i2BEtSpmpbrzQUPoA2ST06IzzcaenyVEHRepHSF3", header=TRUE, row.names=1,  sep=",", dec=".")	   
M <- cor(NC_Associations_Data)

Hyperbolic Visualization

• URL: http://socr.umich.edu/html/Navigators.html

• Tools:

o Java/Jar applet: http://socr.umich.edu/html/navigators/HW/jars/SOCR_HW_Viewer.jar

o JavaScript: http://socr.umich.edu/html/navigators/D3/SOCR_D3_Viewer.html (JSON)

• Data Format

o XML data: http://socr.umich.edu/html/navigators/HW/SOCR_HyperTree.xml

o JSON data: http://socr.umich.edu/html/navigators/D3/xml/SOCR_HyperTree.json

• D3 Visualization

o E:\Ivo.dir\Research\UMichigan\Education_Teaching_Curricula\2015_2016\HS_853_Fall_2015\Modules_docx\Tools\TreeViewer_JS

 treeJS.json

 flareD3.json

• URL: https://github.com/mbostock/d3/wiki/Gallery

• Source code: https://github.com/mbostock/d3

Motion Charts

• Video: http://www.socr.ucla.edu/SOCR_MotionCharts/SOCR_HTML5_MotionChart_Video2.gif

• Java: http://www.socr.ucla.edu/SOCR_MotionCharts/

• HTML5: http://socr.umich.edu/HTML5/MotionChart/

• Activities: http://wiki.socr.umich.edu/index.php/SOCR_MotionCharts

1D/2D/3D signal/area/volume/surface/model/atlas visualization

• 1D: (See R/SOCR Visualization tools above)

• 2D: http://imagej.nih.gov/ij/

• 3D: http://socr.umich.edu/HTML5/BrainViewer/

Supported File Formats:
Volumes (.nii / .nii.gz / .img&.hdr / .mgh / .mgz / .nrrd)
Shapes (.dx / .vtk / .stl / FreeSurfer)
Fibers (.trk)

Trees and Graphs

• Trees/Hierarchies and general Graphs

# Install and load the APE package, needed for the phylogenetic tree rendering (as.phylo)
# install.packages("ape")
library("ape")

Load data

# Data: 02_Nof1_Data.csv
data.1 <- read.table("https://umich.instructure.com/files/330385/download?download_frd=1&verifier=DwJUGSd6t24dvK7uYmzA2aDyzlmsohyaK6P7jK0Q ", sep=",", header = TRUE)
head(data.1)

mydata1

	ID	Day	Tx	SelfEff	SelfEff25	WPSS	SocSuppt	PMss	PMss3	PhyAct
1	1	1	1	33	8	0.97	5.00	4.03	1.03	53
2	1	2	1	33	8	-0.17	3.87	4.03	1.03	73
3	1	3	0	33	8	0.81	4.84	4.03	1.03	23
4	1	4	0	33	8	-0.41	3.62	4.03	1.03	36
5	1	5	1	33	8	0.59	4.62	4.03	1.03	21
6	1	6	1	33	8	-1.16	2.87	4.03	1.03	0

Clustering

hc = hclust(dist(data.1), 'ave') 
# the agglomeration method can be specified "ward.D", "ward.D2", "single","complete", "average" (= UPGMA), "mcquitty" (= WPGMA),"median" (= WPGMC) or "centroid" (= UPGMC)

# (3) Plot clustering diagram
par (mfrow=c(1,1))
# very simple dendrogram
plot(hc)